Practice – Automated Glycan Assembly

Overview and introduction to the different components of AGA system.

An introduction on how to prepare and maintain the core components and solutions of the system.

Tips and techniques for preparing the different solutions required for AGA.

Protocols on distilling and handling TMSOTf.

Protocols on preparing and quantifying linker attachment.

Instructions on what to do after AGA, i.e. how to get your glycan off the resin and how to deprotect it.

Information on analyzing the AGA synthesis such as by MALDI or HPLC.